Loci recovered in 2010, 2012.

RAD libraries were prepared once for each of the seven individuals shown and sequenced separately on an Illumina sequencing platform for each analysis to create technical replicates. Column headings: The number of loci recovered per individual (“2010 loci,” “2012 loci”) is based on the clustering pipeline described in methods. “Increase” is the percent increase in locus number by individual from 2010 to 2012. “Total loci” is the total number of loci that recovered from either the 2010 or 2012 sequencing run for each individual (the union set). “Shared loci” is the total number loci recovered in both the 2010 and the 2012 sequencing run for each individual (the intersection set). “Overlap” is shared loci expressed as a percentage of total loci.