Identifying differentially expressed miRNAs in cancer stem cells of laryngeal squamous carcinoma. Identifying differentially expressed miRNAs in cancer stem cells of laryngeal squamous carcinoma

To understanding the miRNA expression profiling of cancer stem cells of laryngeal squamous carcinoma, the total RNA of CD133+CD44+ laryngeal cancer stem cells (isolated from LSCC cell line TU-177, named TDP), CD133-CD44- cells (TDN) and parental TU-177 (unsorted TU-177 cells, named TPT) was extracted, followed by miRNA sequencing. Differentially expressed miRNAs were identified. Overall design: The CD133+CD44+ cells (TDP) and CD133-CD44- cells (TDN) were isolated from TU-177 cells and cultured. Total RNA was extracted using TRizol reagent (Invitrogen, Carlsbad, CA) following the manufacturer’s instruction. Equal quantities of RNA from 3 samples in one group were mixed. The small RNA library was prepared. The library products were finally sequenced on Illumina HiSeq 2000 platform.