MASH-CDHFD 0,4,8,12 month-Liver-scRNA-seq Raw Data
收藏DataCite Commons2026-03-23 更新2026-05-04 收录
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https://data.mendeley.com/datasets/pzm8mkgvrd/1
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资源简介:
Single-cell RNA sequencing was performed on hepatic tissues from both healthy and MASH mice using the 10x Genomics Chromium platform. Sequencing libraries were processed with CellRanger (v4.0.5) for demultiplexing, read alignment to the GRCm38 reference genome, and initial feature-barcode matrix generation. Subsequent processing and quality control were conducted in R (v4.0.5) using the Seurat package (v5.0.4). Low-quality cells were filtered out based on the following criteria: nFeature_RNA between 300 and 7000, nCount_RNA > 1000 (excluding the top 3% of cells with highest UMI counts), mitochondrial gene percentage (mt_percent) < 10%, and hemoglobin gene percentage (HB_percent) < 3%.
提供机构:
Mendeley Data
创建时间:
2026-03-23



