Transcriptional profiling of striatum and cortex from a mouse model of Huntington's disease (Q140) crossed with Polq heterozygous mice [Polq]

As part of collaboration between UCLA and CHDI, UCLA is creating knockouts (KOs) of 123 genes, implicated in Huntington’s disease (HD) through various computational modeling efforts. Striatum and cortex were isolated from 6-month-old Polq+/- mice crossed with the Q140 knock-in (KI) HD mice and their respective controls. Transcriptomic analysis (RNASeq) was performed on 4 genotypes: WT, Polq+/-, Q140, and Q140 X Polq+/-, 8 replicates per genotype. RNASeq analysis was performed on samples from the striatum and cortex tissues of 6-month-old Polq+/-, Q140 KI, Q140 X Polq+/-, and wild-type mice. N = 8 per group (4F/4M).