<i>ces-1(n703</i>gf<i>)</i>; <i>cya-1(bc416)</i> blocks cell divisions in the ABarp, C and E lineages.

All strains analyzed were homozygous for bcIs66. Lineage analyses were performed for two (wild-type, +/+), three (ces-1(n703gf); cya-1(bc416)) and three (cdc-25.2(RNAi)) embryos raised at 25°C. The ABarp, C and E lineages are shown. Vertical axis indicates approximate time in min after the 1st round of embryonic division, in which P0 divides into AB and P1. In the case of ces-1(n703gf); cya-1(bc416), cell division defects observed in three out of three embryos are depicted in red, defects found in two out of three embryos are depicted in blue, and defects found in one out of three embryos are depicted in orange. In the case of cdc-25.2(RNAi), RNAi was carried out by injection. Since there is some variability of the RNAi effect, the lineage shown here was derived from the embryo with the strongest phenotype (cell division defects observed in this embryo are depicted in green), and the lineages from the other two cdc-25.2(RNAi) embryos are shown in Figure S6. The severe cell division defects in the ABarp, C and E lineages were seen in all three cdc-25.2(RNAi) embryos. The cell death in the ABarp lineage is labeled with the cross. The defects in the C lineage and ABarp lineage result in a defect in the formation of the hypodermis (the mitoses that generate hyp7, hyp5, hyp11, H0, H1, H2, V1, V2, V4, and V6 fail to occur).