Raw data for Figure 3B: Mesoangioblasts and HIDEMs suppress T cell proliferation in a dose dependent manner

CFSE labelled PBMCs (5 x 104/well) were stimulated with anti CD3/CD28 beads (1 x 104/well) (P+B) in the presence or absence of HIDEMs/mesoangioblasts at decreasing ratios (HIDEM/mesoangioblast:PBMC). On day 6 cells were harvested and stained with anti-CD3 antibody and 7AAD, and analysed by flow cytometry. CFSE dilution was analysed on gated CD3+ 7AAD- cells. The percentage of CD3+CFSE dividing cells was calculated for each group and compared to the positive control (P+B), followed by plotting against HIDEM/mesoangioblast:PBMC ratios. Experiments were carried out in duplicates. n=2

CFSE 标记的 PBMCs (每孔 5 x 10^4 个细胞) 在存在或不存在 HIDEMs/中胚层祖细胞的条件下，以递减的比例 (HIDEM/中胚层祖细胞:PBMC) 与抗 CD3/CD28 珠子 (每孔 1 x 10^4 个细胞) (P+B) 进行刺激。在第 6 天，收集细胞并使用抗-CD3 抗体和 7AAD 进行染色，通过流式细胞术进行分析。对 CD3+ 7AAD- 细胞的 CFSE 稀释进行了分析。计算了每个组的 CD3+CFSE 分裂细胞百分比，并与阳性对照 (P+B) 进行比较，随后绘制 HIDEM/中胚层祖细胞:PBMC 比率图。实验重复进行，n=2。