Gene expression microarray analysis on the dentate gyrus of alpha-CaMKII HKO mice

We previously found that mice with heterozygous knockout of the alpha-isoform of calcium/calmodulin-dependent protein kinase II (alpha-CaMKII HKO mice) show various dysregulated behaviors, including cyclic variations in locomotor activity (LA), suggesting that alpha-CaMKII HKO mice may serve as an animal model showing infradian oscillation of mood. We performed gene expression microarray analysis of dentate gyrus from alpha-CaMKII HKO mice. Mice were selected for the sampling such that their LA levels varied among the mice. Dentate gyrus RNA isolated from alpha-CaMKII HKO mice.我们此前发现，钙/钙调蛋白依赖性蛋白激酶II（calcium/calmodulin-dependent protein kinase II）α亚型杂合敲除小鼠（alpha-CaMKII HKO mice）表现出多种行为异常，包括自主活动度（locomotor activity, LA）的周期性波动，这提示alpha-CaMKII HKO小鼠可作为展现情绪亚昼夜节律振荡的动物模型。本研究对alpha-CaMKII HKO小鼠的齿状回开展了基因表达芯片分析。采样时选取了自主活动度存在个体差异的小鼠。实验所用样本为从alpha-CaMKII HKO小鼠齿状回中分离得到的RNA。