Mechanomics AFM Validation - MCF10A-ER-Src

Entry is a part of Mechanomics collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript: Urbanska, M., Ge, Y. et al., De novo identification of universal cell mechanics gene signatures BiorXiv: https://doi.org/10.1101/2021.04.26.441418; first published: 27th April 2021, last updated: 4th Nov 2024 eLife: https://doi.org/10.7554/eLife.87930; first published: 23rd March 2023, last updated: 27th Dec 2024 This dataset contains mechanical measurement of tamoxifen (TAM)-inducible MCF10A-ER-Src cell line (Hirsch et al., 2009, Cancer res.) conducted using atomic force microscopy (AFM). Triplicates of the following experiments are included: • EtOH vs TAM - tamoxifen induction over 72 h vs uninduced control (EtOH) • EtOH-CAV1KD - CAV1 knock-down in EtOH-treated, uninduced cells • TAM-CAV1OE - transient overexpression of CAV1 in TAM-induced cells Measurements were performed using atomic force microscopy (AFM) with a followings setup: Nanowizard 4 (JPK Instruments, Germany). Tip-less cantilevers (Arrow TL1, nominal spring constant k = 0.35–0.45 N m−1, Nanoworld, Switzerland) with a 5-µm bead (PS-R-5.0, microParticles, Germany) glued at the end were used as the indenter. Indentations were performed with a piezo extension speed of 5 μm s−1 to a maximum set force of 2 nN. Following sub-folders are included under each sample/experiment: • calibration - contains data acquired during cantilever calibration (whenever available) • *sample name* - indentation curves in .jpk-force format • *sample name*_processed-yyyy.mm.dd-hh.mm.ss - results of processing of indentation curves Hirsch, H. A., Iliopoulos, D., Tsichlis, P. N. & Struhl, K. Metformin selectively targets cancer stem cells, and acts together with chemotherapy to block tumor growth and prolong remission. Cancer Res. 69, 7507–7511 (2009) https://doi.org/10.1158/0008-5472.CAN-09-2994