RNAseq of Wild Type and pikfyve?8 zebrafish.

Purpose: Phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE) is a newly identified pathogenic gene involved in cataract. This study aimed to investigate the nature and mechanism of vacuoles related to PIKFYVE. Methods: We generated Pikfyve phosphatidylinositol phosphate kinase domain-deficient ( pikfyve?8) zebrafish using CRISPR/Cas9-directed gene editing. The pikfyve?8 homozygous and wild-type zebrafish were subjected to transcriptomic analyses. Results: Transcriptomic analyses revealed 8694 differentially expressed genes (DEGs). Conclusion: Our study represents the first detalis transcriptomic analysis of pikfyve disruption zebrafish. Overall design: We generated Pikfyve phosphatidylinositol phosphate kinase domain-deficient (pikfyve?8) zebrafish using CRISPR/Cas9-directed gene editing. We then performed gene expression profiling analysis using data obtained from RNAseq of three samples respectively in pikfyve?8 homozygous and wild-type zebrafish.