Mutational analysis, complex formation, and enzyme activity of the nsp10/nsp16 complex.
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*as inferred from the crystal structure.**nsp10/nsp16 complex formation as determined using pull-down experiments, SDS-PAGE analysis, and quantitation (see Methods).***The % of MTase activity was determined using filter binding assays (see Methods) relative to wild-type.SAM-BS: S-adenosylmethionine binding site.Mg2+-BS: Magnesium binding site.RNA-BS: RNA binding site.
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2015-12-02
