Experimental set up Study 2.

Shown is the1immunization protocol2the administration route of the vaccine3the amount of adjuvant used per mouse4the volume of the vaccine5the amount of lysate as shown by the protein concentration used per mouse6whether the animals were challenged with H. suis or not and 6the number of animals in each group. (HBSS: Hank’s balanced salt solution, CCR4: CC chemokine receptor 4 antagonist, CT: Cholera toxin, FC: Freund’s complete, IN: intranasally, SC: subcutaneously, SL: sublingually, Neg. control: sham-immunized/not challenged, Pos. control: sham-immunized/challenged,)Experimental set up Study 2.