Bacterial communities of Gilthead seabream (Sparus aurata) larviculture components according to various tank disinfection techniques

In this study, we investigated the effect of tank disinfection technique on the bacterial profile of Gilthead seabream (Sparus aurata) larvae (L) and rearing-water (W) from a commercial fish farm using next generation sequencing of 16S rRNA amplicons. Three different techniques of disinfections were used in the rearing tanks: 1) Chlorination (24hrs) with a set of disinfectants and nebulization with active agent: acetic acid/hydrogen peroxide, Phosphoric acid Sodium hypochlorite/Sodium hydroxide and Didecyl Dimethyl Ammonium Chloride/Polyhexamethylene biguanide hydrochloride (Dis1). 2) Chlorination (24hrs) with Vircon wash, with active agent Potassium peroxymonosulfate/Sodium chloride and 1-week dry period (Dis2). 3) Chlorination (24hrs) with Vircon wash with active agent Potassium peroxymonosulfate/Sodium chloride and 50days dry period (Dis3). For further comparison, the bacterial communities at eggs stage and feed at different larval stages were also included in the investigation.