Anthracnose resistant alfalfa plants

The present disclosure provides alfalfa plants exhibiting broad spectrum resistance to Race 1， Race 2， and Race 5 anthracnose. Such plants may comprise novel introgressed genomic regions associated with disease resistance from Race 1， Race 2， and Race 5 anthracnose. In certain aspects， compositions， including novel polymorphic markers and methods for producing， breeding， identifying， and selecting plants or germplasm with a disease resistance phenotype are provided. Also provided are alfalfa varieties designated as C0416C4164 and H0415C4114. Provided by the invention are the seeds， plants and derivatives of alfalfa varieties C0416C4164 and H0415C4114. Also provided by the invention are tissue cultures of alfalfa varieties C0416C4164 and H0415C4114， and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing alfalfa variety C0416C4164 or H0415C4114 with itself or another alfalfa variety and plants produced by such methods.