Differential expressed genes in EGF treated HTR-8/SVneo versus untreated control by next generation sequencing

The goal of this study is to compare mRNAs expressed by EGF treated HTR-8/SVneo cells to iRNAs expressed in untreated control HTR-8/SVneo cells to identify various genes which play a role during EGF-mediated HTR-8/SVneo cell invasion Overall design: cDNA libraries were made from total RNA of untreated control and 24 h EGF treated samples by TruSeq RNA Sample prep kit. Deep sequencing of cDNA libraries were performed with the help of Illumina HiSeq. RNA-seq reads were aligned to the hg38 genome assembly using Tophat 2.1.1. Tuxedo pipeline was used for transcripts identification and differential analysis.