RNA Sequencing of unc-30(ok613) and wild type (WT) N2 C elegans

Purpose: To uncover immune and longevity genes and pathways that are modulated by the homeodomain PITX1/UNC-30, which plays a vital role in the GABAergic signaling in C elegans Methods: RNA was extracted from synchronized L4 stage unc-30(ok613) and WT animals grown at 20 C using Qiagen extraction kits and following standard methods Results: RNA seq analyses shows enriched and signficant upregulated immune, neuropeptide, ageing and metabolism genes and pathways that are dependent of GABAergic signaling Conclusions: Our study uncovered GABAgergic signaling to be modulator of the innate immunity in C elegans RNA seq profiles of L4-stage unc-30(ok613) and wild type (WT) C elegans animals were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000 platform.