Tumour genotypes in devils with multiple tumours.

Only DFT1 tumours were considered in this analysis, and DFT1–DFT2 coinfection, which occurred in two devils (Devil-812 and Devil-818) in the cohort, is not included. (A) Summary of tumour genotype information for devils hosting more than one sampled tumour. Each animal has an individual ID, and individual tumours sampled from the devil are labelled “T1,” “T2,” etc. “Type” lists various categories of multiple tumour sampling regimens: “multiple tumours,” when several tumours were sampled from an individual devil at a single sampling time and “time-course,” when the same tumour was sampled more than once on different dates. Individuals from which internal metastases were sampled are tagged “metastases collected,” and the tissue location of the relevant tumour(s) is tagged. Each animal is marked as “same” or “different,” depending on if its tumour genotypes are distinguishable using the range of markers that we have assessed (mtDNA and CNVs). Those animals that have different genotypes between multiple tumours are further classified as “polymorphic public,” i.e., all genotypes identified in the animal are shared with tumours in other devils, and “polymorphic private,” i.e., any new variation in the devil’s tumours is found only in that devil. The genetic distance between different tumours within a single host with polymorphic public genotypes are listed and can include coinfection with tumours of different clades, different clade groups, or the same clade group. Each genotype within a devil is given a number, and these are listed in the “pattern” column. Additional metadata, as described in S1 Table, are also provided. (B) Genotype information from cell lines sampled multiple times at different passage or after inoculation into animals. Data are organised as in (A). (C) Summary of data in parts (A) and (B), with underlying data for Fig 1E. (XLSX)