Assessment of Microvessel Isolation Protocol by real-time Quantitative Reverse Transcription Polymerase Chain Reaction to Gauge the Level of Capillary Enrichment

Relative quantification of mRNA transcripts in samples generated from whole murine brain (yellow), vessels collected after enrichment (blue), and microvessels collected after enrichment and filtration (pink). mRNA samples were probed for the relative levels of Acta2, Myh11, Cnn1, Ephb4, Nrf2f, Cldn5, and Pecam1, with TATA-binding protein (Tbp) referenced as the loading control. This analysis provided greater insight into the output from each collection technique with respect to microvessel content.