Data for: An analytical workflow for seed-based correlation and independent component analysis in interventional resting-state fMRI studies.

Rs-fMRI data was acquired from 62 healthy adult (6–8 weeks old, 150–250 g) male Sprague Dawley rats under the effect of combined medetomidine-isoflurane anaesthesia (TE 11 ms and TR 1500 ms). Pre-processing of fMRI data included reorienting the brain into left-anterior-superior (LAS) axes (radiological view), skull-stripping, and upscaling the voxel sizes by a factor of 10. Single-session ICA was then carried out in FSL/MELODIC with the Gaussian kernel filter set to a full-width half maximum (FWHM) of 6.25 mm, a temporal high pass filter cut-off of 100 s and motion correction. FSL/FIX was manually trained by hand-labelling ICA’s decomposition of 60 datasets into signal or noise based on each component’s time-course, frequency, and spatial map. The trained weights training.RData file is provided here. The de-noised fMRI images were then coregistered to their respective T2-weighted images and normalised to a Sprague Dawley brain atlas. Multi-subject temporal concatenation group-ICA as implemented in FSL/MELODIC was carried out to identify functional network templates (provided here). These RSNs can be viewed by overlaying them on the brain atlas supplied. The use of an out-of-sample functional atlas is not recommended for rodent studies if study-specific variations are present. For example, the type and dose of anaesthetic used alter the relative localisation and strength of connectivity within specific networks and even the presence of some RSNs.

本研究通过结合美托明与异氟醚麻醉（TE 11 ms，TR 1500 ms）对62只健康成年斯普鲁恩大鼠（6-8周龄，体重150-250克）进行功能性磁共振成像（fMRI）数据采集。数据预处理包括将大脑重新定位至左前上（LAS）轴（放射学视角）、颅骨剥离以及将体素大小按10倍比例放大。随后在FSL/MELODIC软件中执行单次会话独立成分分析（ICA），设置高斯核滤波器的全宽半高（FWHM）为6.25毫米，时间高通滤波器截止频率为100秒，并执行运动校正。通过手动标记ICA分解的60个数据集，根据每个成分的时间进程、频率和空间图谱，手动训练FSL/FIX，以将信号或噪声区分开来。提供的训练权重文件training.RData即为训练结果。去噪后的fMRI图像随后与各自的T2加权图像进行配准，并归一化至斯普鲁恩大鼠脑图谱。在FSL/MELODIC中执行多受试者时间串联组ICA，以识别功能网络模板（提供于此）。这些区域网络（RSN）可通过叠加至提供的脑图谱中查看。若研究中存在特定变化，不建议使用样本外功能图谱进行啮齿动物研究。例如，麻醉的类型和剂量会改变特定网络内连接的相对定位和强度，甚至某些RSN的存在与否。