tRNA-HydroSeq identifies some tRNA modifications as misincorporations.

A) Scatter plots showing normalized counts of maf1Δ vs. WT (left), and maf1Δ+maf1+ vs. WT (right) cells. B) The tRNASerUGA track profiles from IGV display software for two WT strain replicates (WT-1 and WT-2) showing misincorporations as colored bars. IGV introduced color if ≥15% mismatch is detected relative to the reference gene sequence. Color key: green = A, red = T, orange = G, blue = C. Sequence read counts are indicated on the Y-axis. C) G26 misincorporations for the 36 S. pombe tRNAs that have G at position 26, arranged left to right according to misincorporation level. Error bars indicate standard deviation of 4 samples. D) Heatmap illustrating misincorporation levels in WT, trm1Δ, and trm1Δ+trm1+ over-expression cells.