LncRNA-mRNA expression profile and functional network of vascular dysfunction in endotoxemic rats

In our study, we used a rat model of LPS intervention to extract blood vessels. Differentially expressed LncRNA and mRNA were screened in lps rat blood vessels and normal rat blood vessels by microarray analysis. And through CNC and ceRNA analysis, lncRNA, miRNA, and mRNA regulatory network diagrams were constructed using verified lncRNA. This has the potential to highlight specific molecular functions and mechanisms. Later, we performed functional enrichment analysis and annotation to further explore the importance of lncRNA in vascular cell injury in sepsis. This work helps to reveal the underlying mechanism of vascular cell damage caused by sepsis and provides a new therapeutic target for controlling vascular damage in sepsis. We use high-throughput technologies to screen the lncRNAs and mRNAs in the blood vessel tissues from LPS rats (n=5) compared with Ctrl rats (n=5). Differential expression lncRNAs and mRNAs were confirmed by qRT-PCR. We constructed a CNC and ceRNA network to reveal the core genes in hyposalivation.