File S1 - The Human Antibody Response to the Surface of Mycobacterium tuberculosis

Figures S1 and S2, Tables S1–S8. Figure S1. Reproducibility of human sera on different ELISA antigens: Human plasma was randomly selected from our cohort of 71 individuals and run in duplicate on (A) live M. tuberculosis cell surface (B) whole cell lysate and (C) lipoarabinomannan. Figure S2. Reactivity of human plasma to protein lysates from M. tuberculosis and environmental mycobacteria. Plasma from PPD-negative volunteers (Uninfected) and a randomly selected subset of patients with active TB disease (Active) were assayed against whole cell protein lysates from M. tuberculosis H37Rv (A), M. intracellulare (B), M. avium (C), and M. fortuitum (D). Two-sided p-values by student’s t-test: (*) p≤0.05, (**) p≤0.01, (***) p≤0.001. (E–G) Correlations between log10 antibody titers for M tuberculosis and environmental mycobacteria are displayed for individuals with active disease. Table S1. Univariate correlation between clinical variables and total antibody titers to lipoarabinomannan, cell wall, and secreted proteins in patients with active TB disease (n = 40). Table S2. Univariate correlation between clinical variables and total antibody titers to the live M. tuberculosis surface and to whole cell lysate in patients with latent TB infection (n = 23). Table S3. Univariate correlation between clinical variable and total antibody titers to lipoarabinomannan, cell wall, and secreted proteins for patients with latent TB infection (n = 23). Table S4. Univariate correlation between clinical variables and relative IgG avidity of antibodies to lipoarabinomannan, cell wall, and secreted proteins in patients with active TB disease (n = 40). Table S5. Univariate correlation between clinical variables and relative IgG avidity of antibodies to the live M. tuberculosis surface and to whole cell lysate in patients with latent TB infection (n = 23). Table S6. Univariate correlation between clinical factors and relative IgG avidity of antibodies to lipoarabinomannan, secreted proteins, and cell wall in patients with latent TB infection (n = 23). Table S7. Univariate correlation between cytokine levels in whole blood after Quantiferon-Gold peptide stimulation and total antibody titers to the live M. tuberculosis surface and to whole cell lysate in patients with latent TB infection or active TB disease. Table S8. Univariate correlation between cytokine levels in whole blood after Quantiferon-Gold peptide stimulation and relative IgG avidity to the live M. tuberculosis surface and to whole cell lysate in patients with latent TB infection or active TB disease. (DOCX)