MitoKO: A library of base editors for precise ablation of all protein-coding genes in the mouse mitochondrial genome

We design and apply a highly optimized library of double-stranded DNA deaminase (DddA)-derived cytosine base editors (DdCBEs) - MitoKO - to precisely ablate every mtDNA protein coding gene in the mouse mitochondrial genome Overall design: Base editing of citosine to timine to introduce premature STOP codons in protein-encoding genes of the mouse mtDNA