Implications for Wnt Signaling in XLH Odontogenic Differentiation

X-linked hypophosphatemia (XLH) represents the most common form of familial hypophosphatemia. Although significant advances have been made in the treatment of the bone pathology, patients undergoing therapy continue to experience significantly decreased oral health-related quality of life. The following study addresses this persistent oral disease by further investigating the effect of DMP1 expression on the differentiation of XLH dental pulp cells. Dental pulp cells were isolated from the third molars of XLH and healthy controls and stable transduction of full-length human DMP1 was achieved. RNA sequencing was performed to evaluate the genetic changes following the induction of odontogenic differentiation using either an organic (beta-glycerophosphaet) or inorganic (sodium phosphate) phosphate source. Comparative gene expression profiling analysis of RNA-seq data for dental pulp cells from healthy and XLH patients, with or without constitutive expression of full-length human DMP1, undergoing odontogenic differentiation with either organic (beta-glycerophosphaet) or inorganic (sodium phosphate) phosphate sources.