Listeria infection of melanoma induces nitric oxide and iNOS expression.

aB16F10 murine melanoma or BMDC were infected with LMWT (B16F10-LMWT, BMDC-LMWT) or non-infected (B16F10-NI, BMDC-NI) for 24 hours.bNO produced was measured in cell supernatants. Results are expressed as nmol of NO produced by 105 cells (mean ± SD, PcCell surfaces markers of B16F10 and BMDC infected or not with LMWT were analysed by FACS using the following antibodies: CD11c-PE, iNOS-FITC, CD86-V450 and MHC-II-APC. Samples were acquired using FACSCanto flow cytometer. Results are expressed as the percentages of positive cell (mean ± SD, PListeria infection of melanoma induces nitric oxide and iNOS expression.