Expression of specificity protein 1 and collagen I in primary pterygial tissues

ABSTRACT Purpose: To determine the expression profiles of the transcription factor specificity protein 1 and collagen I in primary pterygial and normal conjunctival tissues, and to explore the role of specificity protein 1 and collagen I in pterygial development. Methods: The pterygial tissues of 20 patients who underwent resection of primary pterygial tissue in our hospital from June 2016 to December 2017 and the conjunctival tissues of 10 patients with enucleation due to trauma were collected. Reverse transcription quantitative-po lymerase chain reaction and western blot analyses were used to detect the relative expression levels of specificity protein 1 and type I collagen at the mRNA and protein levels. Results: The content of specificity protein 1 and collagen I mRNA and protein was significantly greater in primary pterygial tissue than it was in conjunctival tissue (p&lt;0.05). There was a positive correlation between the mRNA and protein levels of specificity protein 1 and collagen I in primary pterygial tissues (protein: r=1, p&lt;0.05; mRNA: r=1, p&lt;0.05). Conclusion: Specificity protein 1 and collagen I are expressed in normal conjunctival and pterygial tissues, but expression is significantly greater in the latter. Specificity protein 1 and collagen I may be involved in the regulation of the development of primary pterygium.摘要 目的：明确转录因子特异性蛋白1（specificity protein 1）与I型胶原蛋白（type I collagen）在原发性翼状胬肉（primary pterygium）及正常结膜组织中的表达谱，并探讨特异性蛋白1与I型胶原蛋白在翼状胬肉发生发展中的作用。 方法：收集2016年6月至2017年12月于我院行原发性翼状胬肉切除术的20例患者的翼状胬肉组织，以及因外伤行眼球摘除术的10例患者的结膜组织。采用逆转录实时定量聚合酶链反应（reverse transcription quantitative-polymerase chain reaction）与蛋白质印迹（western blot）分析技术，检测特异性蛋白1与I型胶原蛋白在mRNA及蛋白水平的相对表达量。 结果：原发性翼状胬肉组织中特异性蛋白1与I型胶原蛋白的mRNA及蛋白表达量均显著高于结膜组织（p<0.05）。原发性翼状胬肉组织中，特异性蛋白1与I型胶原蛋白的mRNA及蛋白水平均呈正相关（蛋白：r=1，p<0.05；mRNA：r=1，p<0.05）。 结论：特异性蛋白1与I型胶原蛋白可在正常结膜组织及翼状胬肉组织中表达，但在翼状胬肉组织中的表达量显著升高。特异性蛋白1与I型胶原蛋白可能参与调控原发性翼状胬肉的发生发展。