Mechanosensitive Gene Regulation by Myocardin-Related Transcription Factors is Required for Cardiomyocyte Integrity in Load-Induced Ventricular Hypertrophy

Cardiac-restricted deletion of myocardin-related transcription factors (MRTFs) in adult mice leads to partially penetrant lethality and rapid deterioration in cardiac physiology in response to trans-aortic constriction (TAC). The goals of the RNA-seq are to define the gene programs altered in MRTF-depleted cardiomyocytes compared to control cardiomyocytes 7 days after TAC surgery. Overall design: MRTF double knockout (called "MRTF dKO" or "dKO") and control mice were subject to TAC or sham surgery at 8 weeks of age. Cardiomyocytes were isolated from knockout or control hearts 7 days after surgery and RNA was isolated using Trizol for RNA-seq analysis.