Replication Data for: Near infrared spectra and calibration for detection of malaria infection in Anopheles gambiae (Keele strain)

<p>Anopheles gambiae (Keele Strain) mosquitoes were infected in the lab with cultured Plasmodium falciparum gametocytes (PfN54) to generate oocyst and sporozoite infected vectors. Controls, uninfected mosquitoes, were generated by feeding mosquitoes on the same blood after gametogenesis had occurred which was triggered by dropping the temperature in the glass feeders to below 30 degrees Celsius. After feeding, mosquitoes were kept for 7 and 14 days to allow parasite development after which each individual mosquito was scanned with near infrared spectroscopy (NIRS) and stored at -20 until processed by qPCR (quantitative polymerase chain reaction) for confirmation of infection and quantification of parasite load. The data shared is composed of all the spectra that were collected (in .spc format for GRAMS IQ software) labeled with a unique identifier which links to the STATA files where the mean number of parasite genomes and age for each individual mosquito are listed. The files used to generate the calibration through partial least square (PLS) regression on GRAMS IQ have also been shared (.tfdx) along with the calibration file (.cal) for uploading on IQ Predict software. We have also shared the prediction outputs of the independent samples that were predicted with the calibrations here developed.</p>