Effects of paraquat on SOD1 transgenic flies.

Five day-old male flies expressing SOD1 proteins in motor neurons (A), glial cells (B) and in motoneurons together with glial cells (C) were treated with 20 mM paraquat in fly food (10 flies per vial). The results do not indicate any significant effect of paraquat in SOD1 flies of all genotypes. Total flies uses for D42Gal4>CS, 183; D42Gal4>UAS-dsod1, 194; D42Gal4>UAS-hSOD1A4V, 138; D42Gal4>UAS-hSOD1G85R, 206; D42Gal4>UAS-hSOD1, 231. M1BGal4>CS, 148; M1BGal4>UAS-dsod1, 171; M1BGal4>UAS-hSOD1A4V, 164; M1BGal4>UAS-hSOD1G85R, 144; M1BGal4>UAS-hSOD1, 200. D42+M1BGal4>CS, 148; D42+M1BGal4>UAS-dsod1, 156; D42+M1BGal4>UAS-hSOD1A4V, 177; D42+M1BGal4>UAS-hSOD1G85R, 286; D42+M1BGal4>UAS-hSOD1, 213.

五日龄雄性果蝇，在运动神经元（A）、胶质细胞（B）以及运动神经元与胶质细胞共同表达SOD1蛋白（C）的情况下，经20 mM的联苯胺处理果蝇食物（每管10只果蝇）。实验结果表明，对于所有基因型的SOD1果蝇，联苯胺并未产生显著的效应。总计用于D42Gal4>CS，183；D42Gal4>UAS-dsod1，194；D42Gal4>UAS-hSOD1A4V，138；D42Gal4>UAS-hSOD1G85R，206；D42Gal4>UAS-hSOD1，231；M1BGal4>CS，148；M1BGal4>UAS-dsod1，171；M1BGal4>UAS-hSOD1A4V，164；M1BGal4>UAS-hSOD1G85R，144；M1BGal4>UAS-hSOD1，200；D42+M1BGal4>CS，148；D42+M1BGal4>UAS-dsod1，156；D42+M1BGal4>UAS-hSOD1A4V，177；D42+M1BGal4>UAS-hSOD1G85R，286；D42+M1BGal4>UAS-hSOD1，213的果蝇数量。