Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM
High-density oligo libraries and CRISPR-Cas9 technology have revolutionized large-scale genetic screens, primarily in cell culture. Here, we present novel platforms for multicellular systems that util
Additional file 6: Supplemental_Table_S5. Share of reads with 5′ U (1 U) within the fraction of reads that did not match any other class of coding or non-coding RNA.