Cholesterol in macrophage regulate gene expersion with or without LPS [statin and LPS RNA-seq]

cholesterol reduction in macrophages by methyl-b-cyclodextran (MCD) or statin alters gene expression. When activated by inflammatory stimilus LPS, macrophages responded in a cholesterol specific fashion. Overall design: RNA-seq was performed on rest RAW macrophages, treated with MCD or statin. Similar RNA-seq also performed on macropahges stimulated with LPS. Two replicates were performed for untreated control (ctl),; two replicates for MCD and statin treated cells (MCD and Statin), respectively. Control cells were stimulated with LPS (ctl-LPS), two replicates, and also statin treated cells were stimulated LPS identically (statin-LPS).