A Comparison of Hepatic Gene Expression Between Wild Type and Cgref1-depleted Mice

Previous reports on the functions and physiological effects of secretory protein Cell Growth Regulator with EF-Hand Domain 1 (Cgref1) are limited. Primarily, our group aims to find out if Cgref1 has any major impact on different tissues. Thus, an extensive study has been carried out. Among the range of experiments and observations recorded, we compared the gene expression in the liver tissues between wild type and Cgref1-knockout mice of C57BL/6 strain using high-throughput RNA sequencing (n=3 per group). Particularly, the results revealed that Cgref1-knockout (KO) mice has significantly downregulated expression of several fatty acid biosynthesis genes – acetyl-coA carboxylases 1 (Acaca) and 2 (Acacb), and stearoyl-coA desaturase 1 (Scd1). Such finding provides supportive evidence that Cgref1 may promote de novo lipogenesis in the liver. Overall design: To investigate hepatic gene expression, total RNA was extracted from mouse liver tissues of both wild type and Cgref1-KO groups for RNA sequencing.