Effect of simulated galactic cosmic radiation on mouse lung gene expression

C57LBL/6 mice (age 24 weeks) were exposed to 0,75 Gy of Si ions, 5-ion simplified GCR simulation, or reference gamma rays. Some mice received nicotinamide riboside countermeasure. Lung tissue was collected after 30 days and flash frozen. Mice were irradiated as listed in the samples section. 5-ion simplfieid GCR simulation is defined as 1 GeV 1-H (34.8%), 600 MeV/U 28-Si (1.1%), 250 MeV/U 4-He ((18.0%), 350 MeV/U 16-O (5.8%), 600 MeV/U 56-Fe (1.0%) and 250 MeV 1-H (39.2%). Nicotinamide riboside was administered continuously in drinking water, starrting prior to irradiation, at a concentration of 12 mM.