Microbial regulation of gene expression patterns in lysosome rich enterocyte (LRE) and intestinal epithelial cells

The goal of this study was to characterize the effects of the microbiome on gene expression patterns in lysosome rich enterocytes (LREs) and other intestinal epithelial cells (IECs). Using transgene assisted cell isolation and single cell RNA-sequencing, we characterize all intestinal cells that take up dietary protein. We find that microbes affect expression of bacteria-sensing and metabolic pathways in LREs, and that some secretory cell types also take up protein and share components of protein uptake and digestion machinery with LREs.