Genome Analysis of Marine Animals (KAP220224)

Assignment goal Through genome production, processing, standard map construction and advanced functional analysis of marine animals (including strategic species), the evolution of marine animals/understanding strategies to respond to changes in the marine environment/establishment of sovereignty over marine biological resources Acquire academic/national/social/industrial values ??by discovering new marine biomaterials. Securing key base data for the development of aquatic biotechnology through detoxification of Takifugu obscurus genome and analysis of Takifugu obscurus transcriptome Assignment contents Analysis of Takifugu obscurus genomics 1) Takifugu obscurus DNA Extraction. HMW DNA extraction and QC from the muscle tissue of Takifugu obscurus 2) Libraries creation 3) Pacbio Sequel sequencing 4) Assembly 5) Scaffolding 6) Assembly QC (BUSCO Results) 7) Transcriptome sequencing 8) Annotation 9) Comparison between Takifugu obscurus and other fishes Takifugu obscurus transcriptome analysis 10) RNAseq experiments on salinity effects . Analysis of changes in expression of Takifugu obscurus transcripts according to salinity changes 10) RNAseq experiments of fertilization on salinity effects. Analysis of changes in transcriptome expression for each stage of development under different salinity conditions . Perform RNAseq analysis by extracting RNA by occurrence period Expected Effects and Utilization . Technical aspects . Accumulate genome analysis know-how by analyzing Takifugu obscurus genome, and build genome infrastructure for marine animals . Economic and industrial aspects . Contribute to creating a national economic growth engine and strengthening competitiveness by providing a basis for nurturing various bio industries by utilizing Takifugu obscurus's bioinformation . Improving the possibility of improving new breeds by discovering genes that form special traits in marine animals . Social aspect . Laying the foundation for the conservation of Korea's unique marine animal resources . Contribute to the establishment of strategies to respond to changes in biological resources due to changes in the marine environment How to use . Provide high-quality reference genome of domestic marine animals . Establishment of domestic marine animal infrastructure through genome analysis of Takifugu obscurus and suggest molecular mechanisms related to the evolution, development, and environmental adaptability of marine organisms based on comparative genomes . To devise a method to strengthen marine organisms' ability to respond to the environment by revealing genes related to stress and environmental adaptability, and suggest industrial application possibilities