Transcriptome analysis of cancer stem cells in triple-negative cancer

Cancer stem cells (CSCs) are thought to be the source of drug resistance, metastasis and tumor recurrence in cancer treatment. The goal of this study was to identify the differentially expressed genes between CSCs and non-stem cancer cells (NSCs). RNA sequencing and bioinformatic analyses revealed that 963 genes are differentially expressed (FDR < 0.4) in TNBC CSCs as compared to NSCs. Overall design: Flow cytometry cell sorting was employed to isolate CSCs and NSCs from SUM149 triple-negative breast cancer (TNBC) cells. RNA samples were prepared using an RNeasy mini kit (Qiagen). RNA sequencing was performed to identify transcriptome profilings