The automated processing of data generated by top down proteomics would benefit from improved scoring for protein identification and characterization of highly related protein forms (proteoforms). Her
Protein scores of >29 was calculated for the 5% confidence threshold.MALDITOF MS/MS sequence results of the 70 kDa band detected in the supernatant of regenerated protoplasts of R. oryzae grown in the
Protein bands of interest were cut from a Coomassie G-250-stained SDS-PAGE gel and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The obtained data were screened against availa