Comparison of the effect of induction of AtGDU1 and the constitutive over-expression of the AtGDU genes on the transcriptome of Arabidopsis thaliana

The over-expression of the AtGDU genes in Arabidopsis leads to strong phenotypes, which have in common glutamine secretion by the hydathodes, increased amino acid export from seedlings, early senescence and lesions on the leaves, and small sized-plants. The goal of the study was (1) to describe the primary effects of the AtGDU1 overexpression on the transcriptome using a dexamethasone-inducible construct, and (2) to compare the transcriptomes of plants overexpressing AtGDU1 to AtGDU6, in order to identify the differences in the function of each of the genes. The 6Op-LHGRn system was used for inducible expression of AtGDU1. The AtGDU1 to AtGDU6 genes were expressed under the control of the CaMV 35S promoter in wild type Col plants. All lines were brought to homozygosity. All plants were grown in parallel on soil for 4 weeks in a growth chamber. Dex-GDU1 and parental 4c-S7 lines were sprayed with 100 µM dexamethasone and leaf samples were collected 7 and 25 hours after. Leaves from the Col and the 35S-GDU lines were collected at the same time. Total RNA were extracted and analyzed by RNAseq. Each library was sequenced twice (L001 and L002, or L003 and L004), with 100 bp paired-end each time (R1 and R2). Information for each sample can be found in the GSE198853_CuffDiff_DIFF.xlsx file below. Each sequencing run is represented by a separate SRR.