Effect of anaerobic digestion of simple sugar amended dairy manure on microbial profiling

Anaerobic digestion of animal manure plays a crucial role in protecting the environment as a waste disposal from food animal production facilities. in addition, it provides a biogas that can be used as a green energy source. Anaerobic digestion is the result of microbial actions in the absence of air. The addition of simple sugars to animal manure may increase biogas production by affecting the microbial profiles in the manure. The objective of this experiment was to evaluate the effect of adding simple sugars to animal manure on microbial profiling during anaerobic digestion. Increasing concentrations of glucose, lactose and sucrose were added to dairy manure, and incubated at 30C for 28 days. Pooled samples of four independent experiments with five concentrations for each sugar type collected 28 days after incubation, as well as a pooled sample of fresh dairy manure samples used for each experiment were sequenced for 16S rRNA. Bioinformatics analysis was performed to compare the microbial profiles of the anaerobically digested sugar amended dairy manure. The publication associated with this project presents the results of this experiment.