SASP-related signals and SASP gene expression profiles in senescent cells after 18β-GA treatment.

The accumulation of senescent cells contributes to age-related organ degradation and susceptibility to chronic diseases. These cells exhibit the senescence-associated secretory phenotype (SASP), affecting cellular and overall aging. 18β-GA has anti-inflammatory effects。RNA-seq was used to analyze the changes in SASP genes and signaling pathways in senescent cells after 18β-GA treatment. 18β-GA can effectively inhibit the activation of IL6 downstream signaling pathways and reduce the expression of SASP-related genes in senescent cells. To detect the effect of GA on SASP in senescent cells. Replicatively senescent IMR-90 cells were incubated with or without 18β-GA (50 μM) for 0 h, 2 h, 4 h, 8 h, 12 h, 24 h. Collect cells and analyze using RNA-seq.