Transcriptome sequencing of axillary buds of switchgrass Pvtb1 mutant and WT plant.

It was shown in the expression database that Pvtb1 genes in switchgrass expressed highly in axillary buds (Panicum virgatum v1.0, DOE-JGI, http://phytozome.jgi.doe.gov/). Thus, we extracted RNAs from axillary buds of tillers. The mutant 52-1 with highly enhanced tiller production and the WT plant (WT-1) generated from the same callus line from which the mutant 52-1 was obtained were chosen for mRNA-seq. RNA-seq analysis revealed a complex regulatory network potentially regulating tillering in switchgrass and provided some clues to the pathways of Pvtb1 genes.

在表达数据库中，研究表明，在 switchgrass 中，Pvtb1 基因在腋芽中高表达（Panicum virgatum v1.0, DOE-JGI, http://phytozome.jgi.doe.gov/）。因此，我们从分蘖的腋芽中提取了 RNA。选取了具有高度增强的分蘖生产能力的突变体 52-1 和由产生突变体 52-1 的相同愈伤组织线培养出的野生型植物（WT-1）进行 mRNA-seq。RNA-seq 分析揭示了一个复杂的调控网络，该网络可能调节 switchgrass 的分蘖，并为 Pvtb1 基因的途径提供了一些线索。