MS-based identification of small proteins in Campylobacter jejuni

The Gram-negative bacterium Campylobacter jejuni is a widespread food-borne pathogen. Knowing the proteins encoded by the bacterial pathogen and how their expression is regulated is essential to understand how it survives, colonizes, and causes diseases. The present study focusses on small proteins (≤ 50-100 amino acids) translated from small open reading frames (sORFs). These poorly annotated components of the genome show emerging roles in bacterial physiology and virulence. Here, the proteome of C. jejuni during exponential growth in a complex medium was analyzed with the proteogenomics workflow presented in Fuchs et al., 2021 with the special focus on small proteins . In combination with different Ribo-Seq approaches new insights into the coding potential of the genome of C. jejuni are provided.