Microarray data of prepubertal, peripubertal, and adult oocytes, and from GV and MII oocytes matured in vivo or in vitro

Expression data from prepubertal, peripubertal, and adult derived mouse oocytes, and from germinal vesicle (GV), in vivo matured, and in vitro matured mouse oocytes. Oocytes derived from prepubertal females, or oocytes matured in vitro, are less developmentally competent compared to adult derived, or in vivo matured, oocytes, indicated by decreased embryonic development. One potential mechanism for decreased developmental potetential in prepubertal or in vitro matured oocytes is inadequate or inappropriate RNA degradation during oocyte maturation (progression from GV to MII). To investigate mechanisms involved in establishing oocyte cytoplasmic maturation and developmental competence, Affymetrix GeneChip microarrays were used. Keywords: Oocyte developmental competence The study encompassed three experimental designs using female B6D2F1 mice: 1) In vitro matured oocytes were obtained from d20 (prepubertal), d26 (peripubertal), and 7-8 wk old (adult) mice; 2) in vivo and in vitro matured oocytes were obtained from d26 mice; and 3) GV, in vivo matured, and in vitro matured oocytes were obtained from 7-8 wk old mice. RNA was extracted from pools of 150 oocytes and hybridized onto the Affymetrix microarrays.本数据集包含青春期前、围青春期及成年小鼠卵母细胞，以及生发泡（germinal vesicle, GV）型、体内成熟型与体外成熟型小鼠卵母细胞的表达谱数据。相较于成年来源或体内成熟的卵母细胞，青春期前雌性小鼠来源的卵母细胞以及体外成熟的卵母细胞发育潜能更低，该差异可通过胚胎发育率降低得以体现。青春期前或体外成熟的卵母细胞发育潜能降低的潜在机制之一，是卵母细胞成熟过程（即从生发泡到减数分裂II中期（MII）的进程）中RNA降解不充分或异常。为探究调控卵母细胞胞质成熟与发育潜能建立的相关机制，本研究采用了Affymetrix GeneChip基因芯片微阵列进行检测。 关键词：卵母细胞发育潜能 本研究以雌性B6D2F1小鼠为实验对象，共设置三组实验设计：1）从出生后20日龄（青春期前）、26日龄（围青春期）以及7~8周龄（成年）的小鼠体内获取体外成熟的卵母细胞；2）从26日龄小鼠体内获取体内成熟与体外成熟的卵母细胞；3）从7~8周龄小鼠体内获取生发泡型、体内成熟型与体外成熟型卵母细胞。从每150枚卵母细胞的混合样本中提取RNA，并将其与Affymetrix基因芯片微阵列进行杂交。