FOXO3a functions as a transcriptional and co-transcriptional splicing regulator in vascular endothelial cell lines in coronary artery disease

Vascular endothelial cells play an important role in the development of coronary artery disease, their injury leads to coronary heart disease and atherosclerosis. This study aimed to elucidate the role of FOXO3-regulated target gene expression and alternative splicing in vascular endothelial cell injury in coronary artery disease Overall design: We constructed a FOXO3A overexpression model in HUVEC cell line, coupled with RNA-seq sequences, to comprehensively reveal the biological function of FOXO3A in HUVEC cell and analyze the alterations of transcriptional profiles upon FOXO3A overexpression and comparative gene expression profiling analysis of RNA-seq data for HUVEC cells and its OE derivatives.