The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor - Shi perturbation calcium imaging data

All calcium imaging experiments that used the temperatures sensitive, synaptic vesicle reuptake inhibitor Shibirets are contained in this zip file. The directories are arranged first by cell type, then by the date of the experiment for that cell type. Each trial has its own .mat files, and each mat file features a matrix containing the raw DF/F signal at each time point for each region of interest (ROIaveMax) and a structure that contains the behavioral data (positionDat). Analysis code can be found at https://github.com/DanTurner-Evans/BiologicalRingAttractor-CodeRelated materials in the collection (https://doi.org/10.25378/janelia.c.5179721):Neuron skeletons and synapses: https://doi.org/10.25378/janelia.12497756One color calcium imaging data: https://doi.org/10.25378/janelia.12490373Kir perturbation calcium imaging data: https://doi.org/10.25378/janelia.12490325Two color calcium imaging data: https://doi.org/10.25378/janelia.12490274Behavioral data for the perturbation experiments: https://doi.org/10.25378/janelia.12490070Dataset supports the publication:Turner-Evans, D. B., Jensen, K. T., Ali, S., Paterson, T., Sheridan, A.,
Ray, R. P., Wolff, T., Lauritzen, J. S., Rubin, G. M., Bock, D. D.,
& Jayaraman, V. (2020). The Neuroanatomical Ultrastructure and
Function of a Biological Ring Attractor. Neuron, 108(1), 145-163.e10.
https://doi.org/10.1016/j.neuron.2020.08.006AbstractNeural representations of head direction (HD) have been discovered in many species. Theoretical work has proposed that the dynamics associated with these representations are generated, maintained, and updated by recurrent network structures called ring attractors. We evaluated this theorized structure-function relationship by performing electron-microscopy-based circuit reconstruction and RNA profiling of identified cell types in the HD system of Drosophila melanogaster. We identified motifs that have been hypothesized to maintain the HD representation in darkness, update it when the animal turns, and tether it to visual cues. Functional studies provided support for the proposed roles of individual excitatory or inhibitory circuit elements in shaping activity. We also discovered recurrent connections between neuronal arbors with mixed pre- and postsynaptic specializations. Our results confirm that the Drosophila HD network contains the core components of a ring attractor while also revealing unpredicted structural features that might enhance the network’s computational power.

本压缩文件中包含所有使用温度敏感型突触囊泡重摄取抑制剂 Shibirets 进行的钙成像实验。目录首先按细胞类型排列，然后按该细胞类型的实验日期排序。每个试验都配有单独的 .mat 文件，每个 mat 文件包含每个感兴趣区域（ROIaveMax）在每个时间点的原始 DF/F 信号矩阵以及包含行为数据（positionDat）的结构。分析代码可在 https://github.com/DanTurner-Evans/BiologicalRingAttractor-Code 找到。该收集相关材料（https://doi.org/10.25378/janelia.c.5179721）包括：神经元骨架和突触：https://doi.org/10.25378/janelia.12497756；单色钙成像数据：https://doi.org/10.25378/janelia.12490373；Kir 混扰钙成像数据：https://doi.org/10.25378/janelia.12490325；双色钙成像数据：https://doi.org/10.25378/janelia.12490274；干扰实验的行为数据：https://doi.org/10.25378/janelia.12490070。数据集支持以下出版物：Turner-Evans, D. B., Jensen, K. T., Ali, S., Paterson, T., Sheridan, A., Ray, R. P., Wolff, T., Lauritzen, J. S., Rubin, G. M., Bock, D. D., & Jayaraman, V. (2020). 生物环形吸引子的神经解剖学超微结构和功能。神经元，108(1)，145-163.e10。https://doi.org/10.1016/j.neuron.2020.08.006摘要：在许多物种中已经发现了头部方向（HD）的神经表征。理论研究表明，与这些表征相关的动力学是由称为环形吸引子的循环网络结构生成、维持和更新的。我们通过进行基于电子显微镜的回路重建和果蝇模式动物头部方向系统中鉴定细胞类型的 RNA 分析，评估了这一理论的结构-功能关系。我们确定了在黑暗中维持 HD 表征、动物转身时更新它以及将其与视觉线索相连的假设性基元。功能研究为单个兴奋性或抑制性回路元素在塑造活动中的假设性作用提供了支持。我们还发现了具有混合前突触和后突触特殊化的神经元树突之间的循环连接。我们的结果证实了果蝇 HD 网络包含环形吸引子的核心组件，同时也揭示了可能增强网络计算能力的未预料到的结构特征。