Genome-wide SNP datasets for the non-native pink salmon in Norway

Effective management of non-indigenous species requires knowledge of their dispersal factors and founder events. We aim to identify the main environmental drivers favouring dispersal events along the invasion gradient and to characterize the spatial patterns of genetic diversity in feral populations of the non-native pink salmon within its epicentre of invasion in Norway. We first conducted SDM using four modelling techniques with varying levels of complexity, which encompassed both regression-based and tree-based machine-learning algorithms, using climatic data from the present to 2050. Then we used the triple-enzyme restriction-site associated DNA sequencing (3RADseq) approach to genotype over 30,000 high-quality single-nucleotide polymorphisms to elucidate patterns of genetic diversity and gene flow within the pink salmon putative invasion hotspot. We discovered temperature- and precipitation-related variables drove pink salmon distributional shifts across its non-native ranges, and ..., 3RAD library preparation and sequencing: We prepared RADseq libraries using the Adapterama III library preparation protocol of Bayona-Vásquez et al., (2019; their Supplemental File SI). For each sample, ~40-100 ng of genomic DNA were digested for 1 h at 37 °C in a solution with 1.5 µl of 10x Cutsmart® buffer, 0.25 µl (NEB®) of Read 1 enzyme (MspI) at 20 U/µl, 0.25 µl of Read 2 enzyme (BamHI-HF) at 20 U/µl, 0.25 µl of Read 1 adapter dimer-cutting enzyme (ClaI) at 20 U/ µl, 1 µl of i5Tru adapter at 2.5 µM, 1 µl of i7Tru adapter at 2.5 µM and 0.75 µl of dH2O. After digestion/ligation, samples were pooled and cleaned with 1.2x Sera-Mag SpeedBeads (Fisher Scientiifc™) in a 1.2:1 (SpeedBeads:DNA) ratio, and we eluted cleaned DNA in 60 µL of TLE. An enrichment PCR of each sample was carried with 10 µl of 5x Kapa Long Range Buffer (Kapa Biosystems, Inc.), 0.25 µl of KAPA LongRange DNA Polymerase at 5 U/µl, 1.5 µl of dNTPs mix (10 mM each dNTP), 3.5 µl of MgCl2 at 25 mM, 2.5 µl of iTru5 primer at ..., , # Genome-wide SNP datasets for the non-native pink salmon in Norway

The complete single nucleotide polymorphisms (SNPs) dataset underwent several filtering steps, including thinning SNPs to a density of one SNP per kilobase, removing closely related individuals, eliminating candidate paralogous regions of the genome (known as multi-site variants or MSVs), and excluding SNPs within non-chromosomal scaffolds. This resulted in a final panel of 43,719 polymorphic SNPs, with a genotyping rate of 0.98 and a sample size of 73 individuals. We eliminated all SNPs that were potentially influenced by selection, as determined by two genome scans for outlier tests. As a result, we obtained a final dataset consisting of 33,860 SNPs that were considered to be neutral. From this dataset, we derived a SNP subset dataset of 250 'diagnostic' SNPs with the highest locus-specific *F*ST.

## Description of the data and file structure

**The ‘neutral’ full-SNP dataset:** OGO-3RAD-D2-NEUTRAL-SNPS.vcf
**NOTE:*...