Data by MS and ER.xlsx

In order to understand the impacts of repeated acute stress and brain serotonin on behavioral adaptations of divergent phenotypes of the olive flounder, <em>Paralichthys olivaceus</em>, we first determined the effect of 5-HT suppression and repeated acute stress on physiological and behavioral changes in bold and shy <em>P. olivaceus</em> separately. Secondly, we measured the effect of 5-HT suppression on repeated acute stress in bold and shy <em>P. olivaceus</em>. Subsequently, we determined the effect of 5-HT and 5-HT1A antagonist WAY-100635 on the activity of Dahlgren cells. Lastly, we determined the effect of 5-HT on the relative expression of 5-HT1A and CRH mRNA in CNSS. All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Shanghai Ocean University (Shanghai, China) and in compliance with the Guidelines on Ethical Treatment of Experimental Animals established by the Ministry of Science and Technology, China.