Replication Data for: "Bee venom exhibits anti-cancer effects on tongue carcinoma cells by arresting cell cycle, inducing apoptosis, and suppressing cell migration"

Objective: Tongue squamous cell carcinoma (TSCC) is a challenging type of oral cancer due to its aggressiveness and resistance to treatment. This in vitro study investigated the potential sole anticancer effect of Honey-Bees Venom (BV) as a mixture of bioactive compounds with potential anticancer properties on the human (TSCC) cell line. Methodology: The cytotoxic effect of serial concentrations of BV (0.01-100 µg/mL) on the cultured human (TSCC) cell line (HNO-97) was determined to calculate the half-maximal inhibitory concentration (IC50) value. Group I/BV included cells treated with (IC50) of (BV), and Group II/Control received no treatment; both were incubated for 48 hours. The apoptotic effect of BV was evaluated through the Annexin V assay and the BAX and BCL-2 gene expression. Cell cycle assay assessed the (BV) effect on cells’ viability, proliferation, and division, and the Transwell migration assay was used to demonstrate the potential impact of (BV) on cell migration. Results: BV showed dose-dependent cytotoxicity and anti-proliferative activity on HNO-97 cells (IC50:12.96 μg/mL). The treated group exhibited cell cycle arrest, suppression in cell migration, and a significantly lower BCL-2 gene expression (p=0.001) and higher BAX gene expression (p=0.03) compared to the untreated group. Conclusion: Bee Venom demonstrated an anticancer effect on human tongue squamous cell carcinoma cells through the induction of apoptosis and inhibition of cell migration, demanding further preclinical investigations of BV as an alternative to current tongue carcinoma therapies. (2025-06-23)