RNA-seq of two NUE-contrasting tomato (Solanum lycopersicum) genotypes after short-term N resupply

We performed the RNA sequencing of two tissues (root and shoot) from two tomato genotypes (Regina Ostuni and UC82) under two N concentrations (0.5 mM and 10mM) added as Ca(NO3)2. Plants were grown in an aerated hydroponic system containing a complete Hoagland solution and grown for 10 days. 20 days old plants were then starved for 5 days (0 mM NO3-) and and then resupplied with low (LN; 0.5 mM) and high (HN; 10 mM) NO 3 - concentrations. Sampling was performed after N starving (0h), at 8 hour after N resupply (8h) and at 24 hours after resupply (24h).