Pervasive transcription-dependent chromatin remodeling influences the replication initiation program. Pervasive transcription-dependent chromatin remodeling influences the replication initiation program

We report here the importance of pervasive transcription in the regulation of replication origins activity in S. cerevisiae. Replication profiles and chromatin profiles were obtained by BrdU-seq and MNase-seq respectively. Overall design: BrdU-seq was performed 70min after release from G1-phase at 18°C in Nrd1-AA, Nrd1-AA dSet2 and Ysh1-AA strains. MNase-seq was performed after 1h of Rapamycin treatment in Nrd1-AA cells.