Reference compounds for characterizing cellular injury in high-content cellular morphology assays

This data accompanies the manuscript "Reference compounds for characterizing cellular injury in high-content cellular morphology assays". Abstract: Robust, generalizable approaches to identify compounds efficiently with undesirable mechanisms of action in complex cellular assays remain elusive. Such a process would be useful for hit triage during high-throughput screening and, ultimately, predictive toxicology during drug development. We generated cell painting and cellular health profiles for 218 prototypical cytotoxic and nuisance compounds in U-2 OS cells in a concentration-response format. A diversity of compounds causing cellular damage produced bioactive cell painting morphologies, including cytoskeletal poisons, genotoxins, nonspecific electrophiles, and redox-active compounds. Further, we show that lower quality lysine acetyltransferase inhibitors and nonspecific electrophiles can be distinguished from more selective counterparts. We propose that the purposeful inclusion of cytotoxic and nuisance reference compounds such as those profiled in this Resource will help with assay optimization and compound prioritization in complex cellular assays like cell painting. This dataset is composed of four parts: (1) List of extracted features from cell painting experiments. (2) Processed live-cell imaging data. (3) Processed intracellular glutathione data. (4) ALARM NMR and UPLC-MS data for KAT inhibitors.